Optimization of collagen extraction process from skin of stingray (rajiformes)
Corressponding author's email:
lucnt@hcmute.edu.vnKeywords:
collagen, stingray skin, extracted, process optimization, acetic acidAbstract
Collagen is widely used in the manufacture of food, pharmaceutical and cosmetic products. Collagen from fish skin has many advantages over other collagen sources, hence, the research of collagen from stingray skin is of interest. The process of cleaning and extracting collagen from stingray skin has been investigated and optimized to create good collagen quality from stingray skin. The optimal conditions of collagen extraction were determined, with three independent factors: acetic acid, solid-to-liquid ratio and extraction time which all affect the extraction efficiency of stingray skin. The optimal conditions to obtain the highest extraction efficiency were determined at acetic acid content of 0.578M, solid-to-liquid ratio of 1/11, and extraction time of 53 hours. Under these optimal conditions, the extraction efficiency reached the highest level of 72.29%. This result is the scientific basis for selecting the optimal conditions for collagen processing from the stingray skin byproducts with the highest quality, creating collagen with white color, and soft fiber structure to meet the commercial collagen quality.
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References
Bae I, Osatomi K, Yoshida A, Osako K, Yamguchi A, Hara K., 2008. Biochemical properties of acid-soluble collagen extracted from the skins of underutilised fishes. Food chemistry, 108, 49–54.
Canh N., 2004. Planning Experiments. 3rd Edn., Published by VNU HCMC, Vietnam
Hashim, P., et al. 2015. Collagen in food and beverage industries. International Food Research Journal 22(1): 1-8.
Hulmes D.J., 2002. Building collagen molecules, fibrils, and suprafibrillar structures. J. Struct. Biol., 137(1-2), p. 2–10
Nagai T, Suzuki N., 2000. Isolation of collagen from fish waste material – skin, bone and fins. Food Chemistry, 68, 277-281
Nagai T, Araki Y, Suzuki N., 2002. Collagen of skin of ocellate puffer fish (Takifugu rubripes). Food Chemistry, 78, 173–177
Nguyen Tien Luc, Le Hoang Du, Nguyen Tan Dung., 2013. Optimization of the Smoking Process of Pangasius Fish Fillet to Increase the Product Quality. Advance Journal of Food Science and Technology. 5(2), 206-212
Nguyen Tien Luc, Nguyen Anh Minh., 2014. Research on the factors that enhance the isolation efficiency of collagen from pangasius fish skin. Science and Technogy Journal of Agriculture & Rural Development Vietnam, 21, 78 – 84
N. Yu. Ignat’eva, et al., 2007. Determination of hydroxyproline in tissues and the evaluation of the collagen content of the tissues. Journal of analytical chemistry 62(1): 59-65.
Perumal S, Antipova O, and Orgel JP., 2008. Collagen fibril architecture, domain organization, and triple-helical conformation govern its proteolysis. Proc Natl Acad Sci U S A, 105(8), p. 2824–9
Sadowska M, Kolodziejska I, Niecikowska C., 2003. Isolation of collagen from the skins of Baltic cod (Gadus morhua). FoodChemistry, N81, p. 257–262
Schmidt, M. M., et al., 2016. Collagen extraction process. International Food Research Journal 23 (3): 913-922.
Jae-Ho Hwang, et al., 2007. Purification and characterization of molecular species of collagen in the skin of skate (Raja kenojei). Food Chemistry 100: 921-925.
Jinhan Shon, et al., 2011. Effect of processing conditions on functional properties of collagen powder from skate (Raja kenojei) skins. Food Science Biotechnology 20(1): 99-106.
Zhang M, Liu W, Li G., 2009. Isolation and characterisation of collagens fom the skin of largefin longbabel catfish (Mystus macropterus). Food chemistry, 115, p. 826–831
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