Ethanol microbial sensor based on gluconobacter xylinus immobilized on eggshell membrane
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tiennv@hcmute.edu.vnKeywords:
Gluconacetobacter xylinus, microbial biosensor, etanol, eggshell membraneAbstract
A microbial biosensor using a pH meter and Gluconacetobacter xylinus -immobilized eggshell membrane was developed. The microbial biosensor responds linearly to ethanol in the range 25–250 mM with a response time of 50 s. The optimal loading of bacterial cells on the biosensor membrane was 30 mg (wet weight). The optimal initial pH for the microbial biosensor was pH 7.0. The biosensor was applied to determine the ethanol contents in various alcohol beverages and the results were compared to that obtained by a distillation-titration method. The proposed microbial biosensor is cost-effective, fast and easy to operate device to determine the ethanol content in simple alcohol beverages.
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