Comparing the Efficiency of Various Ethanol Fermentation Methods from Chaetomorpha sp. Residue
Corressponding author's email:
anh.hoangkim@stu.edu.vnDOI:
https://doi.org/10.54644/jte.2025.1589Keywords:
Chaetomorpha sp., Ethanol, SHF method, SSF method, Combined SHF-SSFAbstract
The Chaetomorpha sp. seaweed residue, which is the carbohydrate-rich remainder after protein extraction, has great potential as a raw material for ethanol production. The residue, after protein extraction, was pretreated with H2SO4 acid at high temperatures, then converted into ethanol using several methods: separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and a combined SHF-SSF method. All fermentation methods used a cellulase enzyme system (including a mixture of cellulase Cellic Ctec2 and β-glucosidase Novozyme 188) and the heat-resistant yeast ThermoSacc® (Saccharomyces cerevisiae). The SHF method was performed by converting the raw material into a sugar-containing solution and then fermenting it immediately; the SSF method was carried out by using enzymes for saccharification and yeast for fermentation at the same time; the combined SHF-SSF method was conducted by saccharifying the raw material (using the SHF method), then adding a portion of the seaweed residue and continuing the fermentation process according to the principles of the SSF method. Upon completion, the results show that the fermentation efficiency of the combined SHF-SSF method reached 89.6%, while the SSF and SHF methods achieved efficiencies of 70% and 54%, respectively. The results indicate that the improved method has superior efficiency compared to the two common methods.
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