Comparison of Quenching Kinetics and Mechanism of Tryptophan by Acrylamide and Genistein Studied by Fluorescence Spectroscopy

Các tác giả

Email tác giả liên hệ:

haohm@hcmute.edu.vn

DOI:

https://doi.org/10.54644/jte.2025.1568

Từ khóa:

Fluorescence quenching, Stern-Volmer plot, Tryptophan, Acrylamide, Genistein

Tóm tắt

Fluorescence quenching using acrylamide and genistein as quenchers has been used to investigate the quenching kinetics and mechanism of tryptophan (Trp) monomer. Fluorescence quenching experiments of Trp by acrylamide were revised as references in present work. The in vitro inhibitory potential against α-glucosidase of genistein has been reported in previous literature, suggesting that the contacts of Trp residue of enzyme and genistein are responsible for the inhibitory activity of genistein. Therefore, genistein was selected as a quencher to symmetrically investigate the quenching kinetics and mechanism of Trp monomer in phosphate buffer pH 6.9. Prior to scanning fluorescence intensity of Trp solutions with (F) and without (Fo) quenchers in a wavelength range from 300-450 nm, fluorophore was excited at 295 nm.  Consequently, the bimolecular quenching constants (kq) were graphically extracted from the Stern-Volmer plot of Fo/F versus quencher concentration [Q]. The values of kq for bimolecular quenching of Trp by acrylamide and genistein are 2.2´109 M-1s-1 and 2.0´1012 M-1s-1, respectively. These experimental results indicated that Trp was quenched by acrylamide through a dynamic quenching mode. Compared with acrylamide, the ground state formation, i.e., a static quenching process of Trp was dominant in the presence of genistein.

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Phuong Ho, Ho Chi Minh City University of Technology and Education, Vietnam

Ho Phuong received a B.E. degree in chemical engineering (2012) and an M.E. degree in chemical engineering (2014) from Ho Chi Minh City University of Technology, Vietnam National University, Ho Chi Minh City. She started her Ph.D. thesis in 2014 in the field of nanomaterials and photovoltaic devices at Yeungnam University, South Korea. After receiving his Ph.D. in chemical engineering from Yeungnam University (2018), she went back to Vietnam and joined the Ho Chi Minh City of Technology and Education. She is currently working at the Faculty of Chemical and Food Technology, Ho Chi Minh City University of Technology and Education as a lecturer. E-mail: hophuong@hcmute.edu.vn. ORCID: 0000-0003-2642-2327

Dat Phat Nguyen, Ho Chi Minh City University of Technology and Education, Vietnam

Nguyen Phat Dat was born in 1992 in Ho Chi Minh City, Vietnam. In 2015, he earned a B.Eng. in Chemical Engineering from the Ho Chi Minh City University of Technology. In 2016, he relocated to the USA and started doing photochemical research under the guidance of Prof. Oleg V. Larionov at the University of Texas at San Antonio. He then joined the research group of Prof. Loi H. Do at the University of Houston in 2017. His doctoral work encompassed studies on metal-catalyzed transfer hydrogenation and single-molecule detection using super-resolution fluorescence imaging (SRFM) within living cells. After earning the Ph.D degree in 2022, he joined the Faculty of Chemical and Food Technology at the University City of Technology and Education. E-mail: datnp@hcmute.edu.vn. ORCID: 0000-0002-0634-8994.

Khoi Dinh Dang, Ho Chi Minh City University of Technology and Education, Vietnam

Dang Dinh Khoi was born on 18th October 1975 in Ca Mau City, the foremost south of Vietnam. He received a B.S. degree in Chemical and Food Technology in 1998 and a M.Sc. degree in Chemical Engineering in 2006, both from Ho Chi Minh City University of Technology, Ho Chi Minh City, Vietnam. He earned his Ph.D. degree in Chemical Engineering in February 2018 from the School of Chemical Engineering, University of Ulsan, South Korea. He also worked as a Post-Doctoral Researcher in the Semiconductor Devices Research Lab, Energy Harvest-Storage Research Center, Research Institute for Natural Science, Department of Physics, University of Ulsan, South Korea from October 2021 to December 2022. From 2019 he joined the Department of Chemical Technology, Faculty of Chemical and Food Technology, Ho Chi Minh City University of Technology and Education, Vietnam. His current scientific interests are synthesis of  fluorescent nanomaterials, especially carbon nanodots, and their applications in various fields such as chemical sensing, bioimaging, and Light-Emitting Diodes (LEDs). E-mail: khoidd@hcmute.edu.vn. ORCID: 0000-0003-0094-2535

Hao Minh Hoang, Ho Chi Minh City University of Technology and Education, Vietnam

Hoang Minh Hao was born in 1982 in Nghe An, Vietnam. He began his studies in Chemistry at the Da Lat University, Vietnam in 2001 and obtained the B.S. degree in 2005. After receiving his M.Sc. in 2009 in the field of Natural Products Chemistry from the University of Science, Vietnam National University Ho Chi Minh City. He started his Ph.D thesis in the group of Prof. Guenter Grampp in the field of the photo-induced electron transfer phenomena using fluorescence spectroscopy. After receiving his Ph.D. from the Graz University of Technology, Austria in 2014, he joined the Ho Chi Minh City of Technology and Education, Vietnam and was working as lecturer. From 2018 to 2019 he worked as a postdoctoral researcher in the laboratory of Prof. Rolf Breinbauer, Graz University of Technology, Austria in the field of protein-protein interaction. Since 2019, he has been a lecturer of Organic Chemistry at the Ho Chi Minh City of Technology and Education, Vietnam. He was appointed as Associate Professor in Chemistry in 2023 at the Ho Chi Minh City of Technology and Education. Now, his group is focusing on Organic Synthesis and Fluorescence Applications in Biochemical systems. E-mail: haohm@hcmute.edu.vn. ORCID: 0000-0003-2614-3619.

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Đã Xuất bản

2025-05-28

Cách trích dẫn

[1]
Phuong Ho, Dat Phat Nguyen, Khoi Dinh Dang, và Hao Minh Hoang, “Comparison of Quenching Kinetics and Mechanism of Tryptophan by Acrylamide and Genistein Studied by Fluorescence Spectroscopy ”, JTE, vol 20, số p.h 02, tr 20–25, tháng 5 2025.

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T. 20 S. 02 (2025)

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