Carotenoid Production of Rhodosporodimum Toruloides under Light Irradiation
Corressponding author's email:
dungpk@hcmute.edu.vnDOI:
https://doi.org/10.54644/jte.80.2023.1411Keywords:
Oleaginous yeast, Carotenoid, Light, Carotenoid composition, Rhodosporidium toruloidesAbstract
Carotenoids are widely used as an antioxidant, a precursor to vitamin A, and food colorants in the medical, cosmetic, chemical, food, and feed industries. They were distributed in diverse microorganisms including bacteria, algae, and fungi. Among them, the yeast Rhodosporidium toruloides is convenient for large-scale fermentation due to its unicellular nature and high growth rate. However, many factors influence the biotechnological synthesis of carotenoids. Light is a crucial consideration while making microbial carotenoids. Carotenogenic is a photo-protective technique used by microorganisms to defend themselves from the light that causes oxidative damage. R. toruloides raised their carotenoid productivity under light conditions in this study. Various lights were used to compare the effect of color light in carotenoid productivity. Results showed that R. toruloides produced more carotenoid content when cultured in blue or white light as compared to a red light or in the dark. Besides, results from microarray showed that this light influence was in the transcription gene level, the light irradiation encouraged the formation of antioxidants such carotenoids, causes a protective mechanism against DNA damage and oxidative stress.
Downloads: 0
References
A. V. Popova and A. S. Andreeva, “Carotenoid-Lipid Interactions,” in Advances in Planar Lipid Bilayers and Liposomes, London, U.K.: Elsevier, 2013, ch. 8, pp. 215-236.
H. Yuan, J. Zhang, D. Nageswaran, and L. Li, “Carotenoid metabolism and regulation in horticultural crops,” Hortic. Res., vol. 2, pp. 15036, 2015, doi: 10.1038/hortres.2015.36.
G. Britton, Carotenoids Handbook, 1st ed. Basel, Switzerland: Birkhäuser Basel, 2004.
J. Avalos et al., “Carotenoid Biosynthesis in Fusarium,” J. Fungi, vol. 3, no. 3, p. 39, 2017, doi: 10.3390/jof3030039.
G. I. Frengova and D. M. Beshkova, “Carotenoids from Rhodotorula and Phaffia: yeasts of biotechnological importance,” J. Ind. Microbiol. Biotechnol., vol. 36, pp. 163–180, 2009, doi: 10.1007/s10295-008-0492-9.
D. M. T. H. Tapiero and K.D. Tew, “The role of carotenoids in the prevention of human pathologies,” Biomed. Pharmacother., vol. 58, no. 2, pp. 100–119, 2004, doi: 10.1016/j.biopha.2003.12.006.
B. Kulczyński, A. G. Michałowska, J. K. Cisowska, and D. Kmiecik, “The role of carotenoids in the prevention and treatment of cardiovascular disease – Current state of knowledge,” J. Funct. Foods, vol. 38, pp. 45–65, 2017, doi: 10.1016/j.jff.2017.09.001.
M. Politino, S. M. Tonzi, W. V. Burnett, G. Romancik, and J. J. Usher, “Purification and characterization of a cephalosporin esterase from Rhodosporidium toruloides,” Appl. Environ. Microbiol., vol. 63, no. 12, pp. 4807–4811, 1997.
A. L. Botes, “Affinity purification and characterization of a yeast epoxide hydrolase,” Biotechnol. Lett., vol. 21, no. 6, pp. 511–517, 1999, doi: 10.1023/A:1005500407152.
P. Buzzini, M. Innocenti, B. Turchetti, D. Libkind, M. V. Broock, and N. Mulinacci, “Carotenoid profiles of yeasts belonging to the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus,” Can. J. Microbiol., vol. 53, pp. 1024–1031, 2007, doi: 10.1139/W07-068.
C. Saenge, B. Cheirsilp, T. T. Suksaroge, and T. Bourtoom, “Efficient Concomitant Production of Lipids and Carotenoids by Oleaginous Red Yeast Rhodotorula glutinis Cultured in Palm Oil Mill Effluent and Application of Lipids for Biodiesel Production,” Biotechnol. Bioprocess Eng., vol. 16, pp. 23–33, 2011, doi: 10.1007/s12257-010-0083-2.
D. Y. Lee, T. Kind, Y. R. Yoon, O. Fiehn, and K. H. Liu, “Comparative evaluation of extraction methods for simultaneous mass-spectrometric analysis of complex lipids and primary metabolites from human blood plasma,” Anal. Bioanal. Chem., vol. 406, no. 28, pp. 7275–7286, 2014, doi: 10.1007/s00216-014-8124-x.
G. Y. Wang, Y. Zhang, Z. Chi, G. L. Liu, Z. P. Wang, and Z. M. Chi, “Role of pyruvate carboxylase in accumulation of intracellular lipid of the oleaginous yeast Yarrowia lipolytica ACA-DC 50109,” Appl. Microbiol. Biotechnol., vol. 99, no. 4, pp. 1637–1645, 2014, doi: 10.1007/s00253-014-6236-z.
J. K. Kim, J. I. Kim, N. K. Lee, Y. T. Hahm, M. Y. Baik, and B. Y. Kim, “Extraction of β-carotene produced from yeast Rhodosporidium sp. and its heat stability,” Food Sci. Biotechnol., vol. 19, no. 1, pp. 263–266, 2010, doi: 10.1007/s10068-010-0038-6.
M. R. Concepcion and C. Stange, “Biosynthesis of carotenoids in carrot: An underground story comes to light,” Arch. Biochem. Biophys., vol. 539, no. 2, pp. 110–116, 2013, doi: 10.1016/j.abb.2013.07.009.
J. Hempel, C. N. Schädle, S. Leptihn, R. Carle, and R. M. Schweiggert, “Structure related aggregation behavior of carotenoids and carotenoid esters”, J. Photochem. Photobiol. A Chem., vol. 317, pp. 161–174, 2016, doi: 10.1016/j.jphotochem.2015.10.024.
C. Ungureanu and M. Ferdes, “Evaluation of antioxidant and antimicrobial activities of torularhodin,” Adv. Sci. Lett., vol. 18, no. 1, pp. 50–53, 2012, doi: 10.1166/asl.2012.4403.
H. Sakaki, T. Nakanishi, A. Tada, W. Miki, and S. Komemushi, “Activation of Torularhodin Production by Rhodotorula glutinis Using Weak White Light Irradiation,” J. Biosci. Bioeng., vol. 92, no. 3, pp. 294–297, 2001, doi: 10.1016/S1389-1723(01)80265-6.
P. B. Bhosale and R. V. Gadre, “Production of β-carotene by a mutant of Rhodotorula glutinis,” Appl. Microbiol. Biotechnol., vol. 55, no. 4, pp. 423–427, 2001, doi: 10.1007/s002530000570.
K. D. Pham et al., “Effect of light on carotenoid and lipid production in the oleaginous yeast Rhodosporidium toruloides,” Biosci. Biotechnol. Biochem., vol. 84, no. 7, pp. 1501–1512, 2020, doi: 10.1080/09168451.2020.1740581.
R. W. S. Weber, H. Anke, and P. Davoli, “Simple method for the extraction and reversed-phase high-performance liquid chromatographic analysis of carotenoid pigments from red yeasts (Basidiomycota, Fungi),” J. Chromatogr. A, vol. 1145, no. 1–2, pp. 118–122, 2007, doi: 10.1016/j.chroma.2007.01.052.
P. Davoli, V. Mierau, and R. W. S. Weber, “Carotenoids and fatty acids in red yeasts Sporobolomyces roseus and Rhodotorula glutinis,” Appl. Biochem. Microbiol., vol. 40, no. 4, pp. 392–397, 2004, doi: 10.1023/B:ABIM.0000040911.85242.3c.
Y. Xu and P. J. Harvey, “Carotenoid production by Dunaliella salina under red light,” Antioxidants, vol. 8, no. 5, 2019, doi: 10.3390/antiox8050123.
Z. Yu and R. Fischer, “Light sensing and responses in fungi,” Nat. Rev. Microbiol., vol. 18, 2018, doi: 10.1038/s41579-018-0109-x.
A. Khanafari, K. Tayari, and M. Emami, “Light Requirement for the Carotenoids Production by Mucor hiemalis,” Iran. J. Basic Med. Sci., vol. 11, no. 1, pp. 25–32, 2008.
Z. Zhang, X. Zhang, and T. Tan, “Lipid and carotenoid production by Rhodotorula glutinis under irradiation/high-temperature and dark/low-temperature cultivation,” Bioresour. Technol., vol. 157, pp. 149–153, 2014, doi: 10.1016/j.biortech.2014.01.039.
Downloads
Published
How to Cite
Issue
Section
Categories
License
Copyright (c) 2023 Journal of Technical Education Science
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright © JTE.
