Investigation of total phenolic content and their antioxidant activities of selected citrus seed oils
Corressponding author's email:
tapchikhgkdt@hcmute.edu.vnKeywords:
Citrus seed oil, flavonoid, limonoid, phenolic content, antioxidant activityAbstract
The extraction methods influence significantly total phenolic content, bioactive compositions such as flavonoids, limonoids and antioxidant capacity of citrus seed oils (Da Xanh pomelo, Duong Hong pomelo, Sanh orange, Num lime). Total phenolic content of seed oils obtained by mechanical pressing is higher than that of oils obtained by solvent extraction method. The con- stituent of polar extracts of the oils was identified and semi-quantitated by LC-MS. There were 7 compounds tentatively identified in citrus seed oils, including 3 flavonoids (5,6,7,8,3’,4’- hexa- methyoxyflavone (nobiletin); 3,3’,4’,5,6,7,8-heptamethoxyflavone; tangeretin) and 4 limonoids (deacetylnomilin; limonin; nomilin; obacunone). Citrus seed oils obtained by mechanical pressing also show higher DPPH free radical scavenging activity than oils obtained by solvent extraction do. The data of Sanh orange seed oil reveals that antioxidant activity with % DPPH scavenging of 31.9% at concentration of 100mg Sanh seed oil/mL solution of 70% methanol in water (v/v), equivalent to capacity of 42.8µg BHT/mL
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